The Korean Journal of Zoology (한국동물학회지)
- Volume 30 Issue 2
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- Pages.117-139
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- 1987
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- 0440-2510(pISSN)
Regulation of Cumulus Expansion of Porcine Cumulus-Oocyte Complexes in vitro: Involvement of cAMP and Calcium
한국인에 대한 지문과 장문의 정량적 분석
Abstract
The present experiments were carried out to investigate the mode of cAMP regulation of cumulus expansion in pig. Intracellular level of cAMP in the cumulus cells was modulated by culturing porcine cumulus oocyte complexes (COC's) with forskolin, an adenylate cyclase stimulator and 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor. The role of calcium in the hormone induced cumulus expansion process was also studied. Forskolin in the medium stimulated cumulus expansion from the concentration of 0.01
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