Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 11 Issue 2
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- Pages.127-131
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- 1987
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- 1226-5284(pISSN)
A Study on the Splitting Methods in Mouse and Bovine Embryos
생쥐 및 소 수정란의 분할방법에 관한 연구
Abstract
This study was carried out to obtain the basic information on splitting and culture of mouse and bovine embryos. Two-, four-, eight-, cell and morula mouse embryos were digested with pronase, splitted in vitro by micro-glass needel with hand, and bovine embryos were splitted by micromanipulator. The splitted embryos were cultured under 5% of CO2 gas in air at 37
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