YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Effects of Food Azo Dyes on the Hepatic Microsomal Mixed Function Oxidase System in Rats

식용 Azo색소가 Hepatic Microsomal Mixed Function Oxidase System에 미치는 영향

  • 윤혜정 (이화여자대학교 약학대학) ;
  • 김백화 (이화여자대학교 약학대학)
  • Published : 1987.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The four azo dyes such as Amaranth (FD & C Red No. 2), Tartrazine (FD & C Yellow No. 4), sunset Yellow (FD & C Yellow No. 5) and Allura red (FD & C Red No. 40) are currently employed as a food additives in Korea. In this study, the effects of these azo dyes on the hepatic microsomal mixed function oxidase systems in Rats. (i.e., Cyt. P-450, Cyt. b$_5$, NADPH cyt. c-reductase and azo reductase) were investigated. Furthermore, to determine the relationship among the electron transport systems, each level of azo reductase, Cyt. P-450 and NADPH cyt. c-reductase was measured upon the administration of phenobarbital (known as an inducer of Cyt. P-450), 3-methylcholanthrene (Known as an inducer of Cyt. P-448), CoCl$_2$ (inhibitor on Cyt. P-450) or $CCl_4$ (inhibitor on Cyt. P-450). The results of these studies are as follows; (1) The levels of Cyt. P-450 and Cyt. b$_5$ were decreased upon the administration of these azo dyes. (2) When the level of Cyt. P-450 was decreased, the azo reductase activity was also decreased. (3) These azo dyes did not show any significant effect on the level of NADPH cyt. c-reductase. (4) The administration of 3-methylcholanthrene resulted in the elevation of azo reductase activity. The 3-methylcholanthrene may be responsible for the induction of CO-insensitive electron transport system.

Keywords

References

  1. 食品添加物公典 韓國食品工業協會
  2. 最新食品衛生學 辛孝善;申光淳;定英彩;李容旭
  3. Handb. Exp. Pharmacol. v.28 Reduction Enzymes Gillette,J.R.
  4. Fd. Cosmet. Toxicol. v.18 Reduction of Sulphonated water-soluble azo dyes by micro-organisms from Human Faeces Watabe,T.;Ozawa,N.;Kobayashi,F.
  5. Toxic. appl. Pharmac. v.3 Grice,H.C.;Mannell,W.A.;Allmark,M.G.
  6. Toxic. appl. Pharmac. v.5 Chronic oral toxicity of Ponceau 3R Hansen,W.H.;Davis,K.J.;Fitzhugh,O.G.;Nelson,A.A.
  7. J. Food Hyg. Soc. Japan v.26 Effect of oral Administration of Azo Dyes on Organ weight, Liver components and RNA Synthesis in Rats Makoto Yoshimoto;Isao Tadokoro;Yoichi Tanaka;Tadao Watanabe;Shoji Hatano
  8. Biochem. J. v.80 Ress,K.R.;Rowland,G.F.
  9. Brit. J. Cancer v.19 Rees,K.R.;Rowland,G.F.;Varcoe,J.S.
  10. J. Food Hyg. Soc. Japan v.25 Effect of Coal Tar Dyes on viability and RNA and Protein Synthesis in Isolated Rat Hepatocyte Makoto Yoshimoto;Kuniaki Noda;Shoji Hatano;Tadao Watanabe
  11. J. Food Hyg. Soc. Japan v.24 Effects of oral Administration of Azo Dyes on RNA Synthesis in Rat Liver Nuclei Yoshimoto,M.;Hatano,S.;Watanabe,T.
  12. J. Pharmocol. Exp. Ther. v.136 The Absorption, Fate and Excretion in Rat of the water-soluble Azo Dyes, FD & C Yellow No.6 Radomski,J.L.;Mellinger,T.J.
  13. J. Pharmacol. Exp. Ther. v.118 Cathartic action and metabolism of certain Coal Tar Food Dyes Radomski,J.L.;Deichmann,W.M.B.
  14. Fd. Cosmet. Toxicol. v.12 Implantation and embryonic survival in Rats treated with Amaranth during gestion Khera,K.S.;Przybylski,W.;Mckinley,W.P.
  15. Toxoc. appl. Pharmac. v.28 Teratologic studies with FD & C Red No.2 in rats and rabbits Keplinger,M.L.;Wright,P.L.;Plank,J.B.;Calan,J.C.
  16. Toxic. appl. Pharmac. v.38 A Teratogenicity study with Amaranth in Cats Khera,K.S.;Roberts,G.;Trivett,G.;Geraldine Terry;Carole Whalen
  17. Fd, Cosmet. Toxicol. v.11 Teratology studies on Food Colourings Part Ⅱ. bmbryotoxicity of R salt and Metabolites of Amaranth (FD & C red No. 2) in Rats Collins,T.F.X.;Mckaughlin,J.
  18. Toxicology v.4 A Teratologic study with the dyes Amaranth and Ponceau 4R in mice Larsson,K.S.
  19. Int. Flavours Food Addit. v.6 The Amaranth debate Drake, J.J.P. Bibra
  20. J. Pharm. Pharmacol. v.10 Chronic toxicity stydies on Food Colours. Part Ⅳ.observations on the Toxicity of Tartazine, Amaranth and Sunset Yellow in Rats Mannell,W.A.;Grice,H.C.;Lu,F.C.;Allanark,M.G.
  21. Toxic. appl. Pharmac. v.35 The fate of FD & C Red No.2 and its Metabolite, Naphthionic acid, after different routes of Administration in the Rat Alan,B.P.;Patricia,A.H.;Kirschman,J.C.
  22. Toxicology v.3 Long-Term effects of Dietary Amaranth in Rats Collins,T.F.X.;Keeler,H.V.;Black,T.N.;Ruggles,D.I.
  23. J. Food Hyg. Soc. Japan v.26 Effects of Coal Tar Dyes on oxygen uptake in Mitochondria Isolated from Rat Liver Noda,K.;Yoshimoto,M.;Hatano,S.;Watanabe,T.
  24. J. Pharmacol. Exp. Ther. v.157 Effect of pretreatment of Rabbits with phenobarbital or 3-Methylcholanthrene on the distribution of drug-metabolizing enzyme activity in subfraction of hepatic microsomes Theodore,E.G.;Larry,A.R.;James,R.F.
  25. J. Pharmacol. Exp. Ther. v.155 Further studies on the Metabolism of drug by subfractions of hepatic microsomes Theodore,E.G.;Larry,A.R.;James,R.F.
  26. Journal of Pharmaceutical Sciences v.73 Relationship between Azo Dye structure and Rat Hepatic Azoredutase activity Shargel,L.;Ali,R.B.;Simon,H.K.
  27. 이화여자대학교 대학원 석사 학위 논문 食用 Azo 色素가 肝의 microsomal azo reductase에 미치는 영향과 Flavin의 효과 元享蘭
  28. Biochemical Pharmacology v.21 Kinetic parameters of drug metabolizing enzymes in $Ca^{+2}$-Sedimented microsomes from Rat Liver Cinti,D.L.;Moldeus,P.;Schenkman,J.B.
  29. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  30. J. Biol. Chem. v.239 The Carbon Monoxidebinding Pigment of Liver Microsomes Omura,T.;Sato,R.
  31. J. Biol. Chem. v.239 The Carbon Monoxidebinding Pigment of Liver Microsomes Omura,T.;Sato.R.
  32. Fundamentals of drug metabolism and drug disposition Experiments illustrating Drug Metabolism in Vitro Mazel,P.
  33. J. Biol. Chem. v.237 Microsomal Triphosphopyridine Nucleotide-cytochrome-reductase of Liver Williams,C.H.;Kamin,H.
  34. Biochem. J. v.210 A continuous spectrophotometic determination of hepatic microsomal azo reductase activity and its dependence cytochrome P-450 Mallett,A.K.;King,L.J.;Walker,R.
  35. J. Pharmocol. Exp. Ther. v.120 Enzymatic reduction of prontosil and other azo dyes Fouts,J.R.;Kamm,J.J.;Berodic,B.B.
  36. J. Biol. Chem. v.128 A New Coupling component for sulfanilamide determination Bratton,A.C.;Marshall,E.K.
  37. J. Pharmacol. Exp. Ther. v.158 Further studies on the Metabolism of drugs by subfractions of hepatic microsomes Ⅲ. Effect of the NADPH-Generating system Gram,T.E.;Fouts,J.R.
  38. Molecular Pharmacology v.14 Inhibition of Azoreductase by oxygen Mason,R.P.;Peterson,F.J.;Holzman,J.L.
  39. Burger’s Medicinal Chemistry v.4 Nelson,S.D.
  40. Ann. Rev. Pharmacol. v.11 Anders,M.W.
  41. Pharmacological Review v.19 Conney,A.H.
  42. Federation Proceedings v.35 Liver microsomal Drug-metabolizing enzyme system: Functional components and their properties Anthony,Y.H.Lu
  43. Biochem. J. (proceedings) v.115 Introduction of drug metabolizing enzymes and cytochrome P-450 Long,R.F.
  44. J. Biol. Chem. v.251 Multiple forms of Rat Liver cytochrome P-450 Tomas,P.E.;Ryan,L.D.;Wert,S.B.
  45. Biochem. J. v.182 Multiplicity of induction patterns of rat liver microsomal monooxygenases and other polypeptides produced by administration of various xenobiotics Shrma,R.E.;Cameron,R.G.;Faber,E.;Griffin,M.J.
  46. Biochemical Pharmacology v.22 Effect of riboflavin defficiency on Phenobarbital and 3-Methylcholanthrene induction ot microsomal durg-metabolizing enztmes of the Rat Shargel,L.;Mazel,P.