실험동물 사료의 처리방법별 살균효과와 성분변화

Sterilization and Quality Changes of Laboratory Rodent Diet by Different Treatments

실험동물 먹이의 재래적인 살균방법인 훈증제처리(燻蒸劑處理) 및 가열처리(加熱處理)와 새로운 방법인 방사선(放射線) 조사(照射)와의 살균효과(殺菌效果) 및 사료(飼料)의 이화학적(理化學的) 품질(品質)등에 미치는 영향을 비교 평가해본 결과 $10{\sim}20\;kGy$의 방사선(放射線) 조사(照射)와 $121^{\circ}C$에서 20분간 가열처리(加熱處理)로서 전미생물(全微生物)을 완전(完全) 살균(殺菌)시켰으나 ethylene oxide 처리는 $10^2/g$정도 생존되어 살균이 불충분하였다. 총아미노산 함량은 20kGy 조사구(照射區)가 대조구에 비해 약 5% 정도 감소되었으나 ethylene oxide 처리와 가열처리는 각각 15%와 20%의 감소율을 보였으며 전당 및 무기질 함량은 저리구간에 유의적인 차이를 나타내지 않았다. TBA가(價)는 20kGy 조사구(照射區)와 ethylene oxide 처리구 및 가열처리구에서 다같이 증가되었으나 조사구(照射區)의 pH 값은 매우 안정하였으며 ethylene oxide 처리구와 특히 가열처리구는 감마선 조사구(照射區)에 비해 시료의 색도에서 명도를 저하시킨 반면 적색도를 크게 상승시켜 외관적 품질을 상당히 저하시켰다.

The usual sterilization methods such as fumigation and autoclaving of laboratory rodent diet was compared with a new irradiation treatment in the point of sterilization effect and physico-chemical quality. Under the treatments of $10{\sim}20\;kGy$ ${\gamma}-irradiation$ and autoclaving at $121^{\circ}C$ for 20 min, total microorganisms were eliminated but ethylene oxide fumigation was insufficient to destory them. Total amino acid content was reduced about 5% in 20 kGy radiation treatment compared with control, while in the ethylene oxide fumigation and the autoclaving, the reducing rate was markedly great as 15% and 20%, respectively. Total sugar and mineral contents were not significantly different among treatment among treatment groups. TBA values were increased in all treatments. The pH of irradiated group war stable in comparison with that of ethylene oxide. In the treatments of ethylene oxide and especially autoclaving, overall appearance was decreased to a great extent as a result of decreasing lightness and increasing redness.