메탄올 자화성 Candida boidinii YF-3의 분리와 단세포 단백질(SCP)의 생산

Isolation of Methanol-assimilating Candida boidinii YF-3 and Production of Single Cell Protein

토양, 호수, 개천, 연못, 부때우유, 단무지로부터 메탄올을 자화하는 효모와 세균을 분리하였다. 효모 중 가장 생육이 빠른 균주를 선발하여 동정하고 메탄올 배양에서의 생육특성을 조사하였다. 그 결과 분리된 효모 YF-3은 Candida boidinii로 동정되었다. 이 균주의 특성은 biotin을 요구하며 $C_1$화합물 외에 다른 화합물도 이용할 수 있는 facultative methylotroph로서 최적 pH는 6.0, 최적 온도는 $30^{\circ}C$, 최적 매탄올 농도는 배지의 1%(v/v)였다. 균체 생산량은 건조균체로 2.72g/l 균체 수율은 $0.39\;g\;g^{-1}$로 측정되었다. 균체의 총핵산 함량은 5.9%, 조단백질 함량은 45.5%, 총아미노산 함량은 35.22%도 분석 되었다.

A large number of methanol-assimilating yeasts and bacteria were isolated from samples of soil, sewage, decomposed milk and spoiled sweet-radish pickles. Among the yeasts, one strain was selected and identified as a strain of Candida boidinii. In 1% (v/v) methanol Candida boidinii YF-3 grew well and could grow in as much as 5%. This yeast required boitin for grwoth. Maximum growth was observed at $30^{\circ}C$ and pH 6 in a semisynthetic medium. The productivity was 2.72g dry cells per liter in batch culture with 1%(v/v) methanol and the cell yield for methanol was $0.39\;gg^{-1}$. The specific growth rate was $0.11\;h^{-1}$ and the generation time was 6.4 hours. The protein content of the cell was 45.5% and total nucleic acid content was 5.9%. The amino acid profile was as good as FAO standard for food protein.