The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Protoplast Isolation and reversion from Pleurotus spodoleucus

참느타리버섯의 원형질체(原形質體) 분리(分離) 및 환원(還元)

  • Yoo, Young-Bok (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • Byun, Myung-Ok (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • Go, Seung-Joo (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • You, Chang-Hyun (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • Chang, Kwon-Yawl (Department of Agronomy, College of Agriculture, Gyeongsang National University)
  • 유영복 (농촌진흥청 농업기술연구소 균이과) ;
  • 변명옥 (농촌진흥청 농업기술연구소 균이과) ;
  • 고승주 (농촌진흥청 농업기술연구소 균이과) ;
  • 유창현 (농촌진흥청 농업기술연구소 균이과) ;
  • 장권열 (경상대학교 농과대학 농학과)
  • Published : 1987.03.30
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Abstract

This experiment was undertaken to investigate proper conditions for protoplast formation from Pleurotus spodoleucus. Novozym 234 was the most effective enzyme and a high yield of protoplasts was obtained. Combination of enzymes did not improve this result. Sucrose gave the best result to support the release and maintain the stability of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs. in shaking condition at 120 strokes $min-^1$. When mycelium of P. spodoleucus was cultured for 3 days on mushroom complete agar medium at $27^{\circ}C$, the formation of protoplasts was effective. The mushroom complete agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.

식용버섯의 일종인 참느타리버섯 P. spodoleucus의 유전육종을 위하여 균사체로부터의 원형질체 분리와 분리된 원형질체의 환원에 영향을 미치는 몇가지 요인을 조사한 결과는 다음과 같다. 1. 원형질체 분리에 알맞는 효소로는 Novozym 234이며, 삼투압 조절제로는 0.6 M sucrose, 효소처리 시간은 3 시간이었다. 그리고 균사체 배양일수는 exponential phase인 3 일이었으며, colony의 $cm^2$당 원형질체 생성수는 $3.43{\times}10^{5}ml^{-1}$이었다. 2. 원형질체 환원에 알맞는 삼투압 조절제는 0.6 M sucrose이며, 환원율은 0.01-0.28%였다.

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