한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제19권6호
- /
- Pages.537-546
- /
- 1986
- /
- 0374-8111(pISSN)
- /
- 2287-8815(eISSN)
어류의 장기조직에 분포하는 단백질분해효소에 관한 연구 1. 고등어 유문수조직으로부터 3종의 알칼리성 단백질분해효소의 분리${\cdot}$ 정제
The Proteinase Distributed in the Intestinal Organs of Fish 1. Purification of the Three Alkaline Proteinases from the Pyloric Caeca of Mackerel, Scomber japonicus
-
PYEUN Jae-Hyeung
(Department of Nutrition and Food Science, National Fisheries University of Pusan) ;
- KIM Hyeung-Rak (Department of Nutrition and Food Science, National Fisheries University of Pusan)
- 발행 : 1986.11.01
초록
고등어 유문수조직중에 분포하는 알칼리성단백질분해효소의 정제조건을 검토하고 염석, DEAE-Sephadex A-50 칼럼 크로마토그래피 및 Sephadex G-100 겔 여과에 의해 3종류의 알칼리성단백질분해효소의 분리
In the previous paper(Kim et al, 1986), the alkaline proteinase from the pyloric caeca of mackerel was shown relatively strong activity in the alkaline pH range. Therefore purification of the enzyme has been undertaken to identify the proteolytic enzyme and three alkaline proteinases were isolated by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography and Sephadex G-100 gel filtration. One percent sodium chloride solution was the most effective for the extraction of alkaline proteinase from the pyloric caeca of mackerel. Three alkaline proteinases temporarily designated Enz. A, B and C were isolated from the pyloric caeca of mackerel, and identified to be homogeneous with electrophoresis. The specific activity of the purified Enz. A, B and C was increased to 34, 53 and 37-fold over the crude enzyme solution, respectively. Yield of them was 1.6, 2.1 and
키워드