구기자(枸杞子) 탄저병균(炭疽病菌)의 배양적(培養的) 성질(性質), 영양(營養) 및 탄저병(炭疽病) 발병(發病)에 미치는 약제(藥劑)의 영향(影響)

Cultural Characters, Nutrition of the Colletotrichum spp. Isolated from Anthracnose of Lycium chinense and Effect of Fungicides on Disease Incidence

1. 배지(培地)의 종류(種類)에 따른 구기자(拘杞子) 탄저병균(炭疽病菌)의 생육(生育)을 조사(調査)하였던 바 Colletotrichun dematium 과 C. gloeosporioides 모두 균사생육(菌絲生育)이 V-8 Juice Agar와 Oatmeal Agar 에서 좋았고 분생포자형성(分生胞子形成)은 C. gloeosporioides는 모든 배지(培地)에서 양호(良好)하였으나 C. dematium은 PDA 배지(培地)와 V-8 Juice Agar 배지(培地)에서 양호(良好)하였다. 2. 균사생장최적온도(菌絲生長最適溫度)는 C. gloeosporioides는 $25^{\circ}C$였고 C. dematium은 $28^{\circ}C$였으며 광(光)의 조사(照射)가 2종(種)의 탄저병균(炭疽病菌) 모두 포자형성(胞子形成)올 촉진(促進)하였다. 3. C. gloeosporioides는 질소원(窒素源)으로 Gelatin, 탄소원(炭素源)으로 Dextrin과 Sorbitol 첨가배지(添加培地)에서 생육(生育)이 양호(良好)하였고 C. damatium은 Type간에 차이(差異)가 있었으나 질소원(窒素源)으로 Glycin, Gelatin, 탄소원(炭素源)으로 Dextrin 첨가배지(添加培地)에서 생육(生育)이 좋았다. 4. 11종(種)의 탄저병(炭疽病) 방제약제(防除藥劑)의 방제효과(防除效果)를 조사(調査)하였던 바 Benomyl, Carbendazim, Dithianon, S-3308 L, Folpet 등이 실내시험(室內試驗)과 포장시험(圃場試驗)에서 비교적(比較的) 좋은 방제효과(防除效果)를 보였다.

Mycelial growth of Colletotrichun dematium and C. gloeosporioides isolated from anthracnose of Lycium chinense was favorable on V-8 Juice Agar media and Oatmeal Agar media of the four different media tested. Sporulation of C. dematium was favorable on PDA media and V-8 Juice Agar media and that of C. gloeosporioides was favorable on all media tested. The optimum temperature for mycelial growth of C. gloeosporioides and C. dematium was $25^{\circ}C$ and $28^{\circ}C$, respectively and sporulation of the fungi was stimulated under alternating NUV light and darkness. Of the seven nitrogen sources tested, gelatin was the most favorable for mycelial growth of C. gloeosporioides and glycin and gelatin were favorable for that of C. dematium. Of the eleven carbon sources tested, dextrin and sorbitol were favorable for mycelial growth of C. gloeosporioides and dextrin was the most favorable for that of C. dematium. Among the eleven fugicides tested, Benomyl, Folpet, Dithianon, Carbendazim and S-3308L were found superior for the inhibition of mycelial growth and spore germination of the fungi. They were, also, found superior for the control of anthracnose disease of L. chinense when they were applied to the plants in the field.