Isolation and Characterization of Leptospira Interrogans in Korea, 1985

한국에서 분리된 렙토스피라균의 생물학적 특성에 대한 연구(1985)

  • Park, Kyung-Seok (Department of Microbiology, National Institute of Health) ;
  • Oh, Hee-Bok (Department of Microbiology, National Institute of Health) ;
  • Lee, Myung-Sook (Department of Microbiology, National Institute of Health) ;
  • Sung, Won-Keun (Department of Microbiology, National Institute of Health) ;
  • Park, Mi-Yeoun (Department of Microbiology, National Institute of Health) ;
  • Lee, Yong-Woo (Department of Microbiology, National Institute of Health) ;
  • Kim, Ho-Hoon (Department of Microbiology, National Institute of Health) ;
  • Paik, Sung-Bok (Department of Microbiology, National Institute of Health)
  • Published : 1986.09.30

Abstract

A microbiological study for the isolation and characterization of Leptospira interrogans was performed in an attempt to define the characteristics of Leptospirosis in Korea. The results are summerised as follows. Thirty-five cultures were isolated from 11 patients with leptospirosis and 24 wild rodents captured in Paju area. The isolation rate of Leptospira from wild rodents reached 23.1%. All 35 cultures were identified as Leptospira interrogans by their characteristic morphology and motility in dark field microscopy, pathogenecity in Guinea pig and sensitivity to 8-Azaguanine. Cultures formed diffuse subsurface colonies with hazy margin and were catalase, peroxidase and oxdase positive. The fifty percent lethal dose of isolate HM 4 in Guinea pig was $8.9{\times}10^4$ org. Mouse passage method was sucecssfully applied in maintenance of fresh isolates without any loss of their original virulency.

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