Effects of Temperature, Seed Water Content and Osmoconditioning on Germination and Seedling Elongation of Soybeans

온도.종자수분함량 및 삼투처리가 콩의 발아 및 묘신장에 미치는 영향

  • Rak Chun, Seong (Dept. of Horticulture, Pai-Chai University) ;
  • Many C., Minor (Dept. of Agronomy, College of Agriculture, University of Missouri-Columbia) ;
  • Eun Hi, Hong (Corp Experiment Station, RDA)
  • Published : 1986.12.01

Abstract

Germination and seedling elongation of soybeans [cult. ‘Williams’] were measured at two temperatures (15 and 3$0^{\circ}C$), three seed water contents (30, 50, and 70%), and with and without polyethylene glycol-8000 (PEG) treatments. A split-plot design was used with four replications. Observations were made from two hours to eight days for each treatment combination. Seeds were soaked with 30% PEG solution with 0.2% thiram at 15 C for six days, rinsed with deionized water and dried at 25.5$^{\circ}C$ for three days. Ten treated or untreated seeds were placed on Whatman No. I (9 cm) filter paper in plastic petridishes to which sufficient deionized water was added to adjust seed moisture content to the desired level. The dishes were then sealed with parafilm and placed in a continuous programmed temperature chamber under dark condition. Seedling growth did not occur at either temperature when moisture content was 50 percent or less. Osmo-conditioning with PEG showed positive effects on seedling moisture uptake and seedling growth at 15$^{\circ}C$ but little effect at 3$0^{\circ}C$. Seedling moisture content increased rapidly early in the germination period and reached 60% at 15 C for 56 hours and at 3$0^{\circ}C$ for 28 hours. Seedling growth started when seed moisture reached a critical point of 60% at 15$^{\circ}C$, however, growth started after 20 hours of germination at 3$0^{\circ}C$. Seedlings of soybeans elongated more than four times faster at 3$0^{\circ}C$ than at 15$^{\circ}C$. Water uptake during germination was characterized by two phases in this experiment.

콩 품종(williams)의 발아와 묘신장을 두 온도, 세 종자, 수분함양 및 Polyethylene glycol-800(PEG) 처리와 무처리에서 측정하였다. 분할구배치법 사반복으로 실시하였으며 시료를 2시간부터 8일까지 각처리 조합에서 채취하였다. 콩 종자를 0.2% Thiram을 첨가한 30% PEG 여액에 놓아 15$^{\circ}C$에서 6 일간 침청후 증류수로 선정하여 25.5$^{\circ}C$에서 3 일간 건조하여 10개의 처리 또는 무처리 종자를 plastic petri-dish안의 9 cm 여과지에 놓아 수분함양에 정당한 증류수를 첨가하였다. Parafilm으로 밀봉하여 항온무광조건의 처리 온도에서 발아시켰다. 수분함양이 50% 또는 이하인 처리에서는 두 온도 모두 묘신장이 일어나지 않았다. PEG를 이용한 삼투처리는 15$^{\circ}C$에서는 묘수분 흡수와 체신장간에 정의 상관 효과를 보였으나 3$0^{\circ}C$에서는 거의 없었다. 종자 또는 묘수분함량은 발아기간동안 초기에 급속히 증가하여 15$^{\circ}C$에서는 56시간에, 그리고 3$0^{\circ}C$에서는 28시간에 60%에 도달하였다. 묘신장은 종자수분함양이 15$^{\circ}C$에서는 60%에서 시작되었지만 3$0^{\circ}C$에서는 20시간에 시작되었다. 콩묘는 15$^{\circ}C$에 비하여 3$0^{\circ}C$에서 사배 이상 신장되었다. 발아기간 동안의 수분흡수양상은 이 시험에서는 두 단계로 특정되었다.

Keywords