한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제14권3호
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- Pages.265-270
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- 1986
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Lactobacillus casei 의 세포융합에 관한 연구
Studies on the Protoplast Fusion of Lactobacillus casei
- Baek, Young-Jin (Korea Yakult Institute for Microbiological Research) ;
- Min Yoo (Korea Yakult Institute for Microbiological Research) ;
- Kim, Young-Kee (Korea Yakult Institute for Microbiological Research) ;
- Bae, Hyeong-Suk (Korea Yakult Institute for Microbiological Research) ;
- Kim, Hyun-Uk (Lab of Dairy Technology & Microbiology, College of Agriculture, Seoul National University)
- 발행 : 1986.06.01
초록
L. casei세포의 유전연구를 위한 도구로서 세포융합 기술을 연구하였으며 융합세포(recombinant)를 선발하고 확인하기 위한 유전자 선발표지 인자로서 항생제 저항성이 이용되었고, 항생제 저항성 돌연변이 균주는 nitrosoguanidine을 처리하여 분리하였다. 선발 배지에서 항생제의 적절한 최종 농도는 streptomycin 25
The best conditions for the protoplast fusion of Lactobacillus casei have been searched for in this study. Antibiotic resistance was used as the selective marker for enumerating and selecting the recombinants. Antibiotic resistant mutants were isolated after treating cells with N-methyl-N'-nitro-N'-nitrosoguanidine. High frequency fusion of protoplasts of L. casei strains were obtained in the presence of 40% (wt/vol) polyethylene glycol 4,000 after 1 min at 3