한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제14권2호
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- Pages.139-143
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- 1986
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Ganoderma lucidum 균주에 의한 Laccase의 정제 및 효소적 특성
Purification and enzyme characteristics of laccase from Ganoderma lucidum
초록
The production media and enzymatic characteristics of laccase from Ganoderma lucidum was investigated. Potato dextrose yeast extract media was proved to be the best for laccase production. The enzyme has optimum pH of 6.45km value of 6.71 mM and appeared to be stable at wide pH range. The enzyme was inactivated partially by methanol and ethanol and totally by sodium azide but not at all by acetone. Also the enzyme purification was performed and the data is given.