Induction of Symbiosis between Nostoc muscorum and Cultured Plant Cells I. Effects of Polyamines on the Association of N. muscorum with Tobacco and Soybean Cultured Cells

Nostoc muscorum과 식물배양세포의 공생유도에 관한 연구 I. Polyamine이 N. muscorum과 담배 및 대두 배양세포의 혼합배양에 미치는 영향

An experimental system for the possibility of association between cultured plant cells and a cyanobacterium (N. muscorum) was investigated. The association was established in nitrogen-free medium, and nitrogen source was provided by N. muscorum through its own nitrogen fixation. N. muscorum was distributed on the outer part and intercellular spaces of the cultured plant cells. Polyamines were treated to the nitrogen-free medium to improve the association. Polyamines increased nitrogenase activity and protein amount in the association between N. muscorum and the cultured plant cells, as compared to the association without polyamines. Especially, spermine showed an increasing effect on the nitrogenase activity and the protein content in the association of tobacco and N. muscorum, while spermidine showed similar effect in case of soybean and N. muscorum.