Journal of Plant Biology
- 제29권1호
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- Pages.1-10
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- 1986
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- 1226-9239(pISSN)
전기장하에서의 담배 및 완두 원형질체 융합
Electrofusion of Tobacco and Pea Protoplasts
초록
Intra- and inter-specific protoplast fusion of tobacco (Nicotiana tabacum cv. Virginia 115) and pea (Pisum sativum cv. Sparkle) were carried out in highly inhomogeneous alternating electric fields. Under the electric field of alternating current (AC, sine wave), 600 V/cm and 800 kHz for tobacco protoplast, and 600 V/cm and 700 kHz for pea protoplasts, the protoplasts were aggregated in pearl chains. Intra-specific protoplast fusions were most effectively induced within the aggregates of tobacco and pea, respectively, by the additional application of a single high field pulse of direct current (DC, square wave) at 1 kV/cm for 50
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