Physiological Studies on the Formation of Hairy Root by the Agrobacterium rhizogenes 1. Relationships between LAA content and morphological characteristics in carrot infected by A. rhizogenes

Agrobacterium rhizogenes에 의한 Hairy Root 형성에 대한 생리학적 연구

Inoculation of carrot discs with Agrobacterium rhizogenes harboring Ri plasmid resulted in transformation of cells, as revealed by the tumors and hairy roots arising from them. Measurements of IAA content using HPLC indicate that it is higher in tumors and inoculated tissues than in uninoculated tissue. A lot of meristemoids and vessel elements formed I tumor tissue and the hairy roots differentiated from meristemoids. IAA content in tumor tissues is decreased with hairy root and vessel elements formation from them. Formation of wound callus in uninoculated tissues resulted on wound healing but no formation of vessel elements and hairy roots. Tumor tissues show continuous growing on hormone free medium.