Korean Journal of Microbiology (미생물학회지)
- Volume 24 Issue 1
- /
- Pages.1-6
- /
- 1986
- /
- 0440-2413(pISSN)
- /
- 2383-9902(eISSN)
Isolation and identification of tumor-inducing (Ti) Plasmids
식물종양유도 플라스미드의 분리 및 동정
Abstract
Tumor-inducing (Ti) plasmid which may be used in genetic engineering of higher plants, was isolated from Agrobacterium tumefaciens in Korea. Among the 7 strains of A. tumefaciens isolated from various regions in Korea, KU12, KU13, KU14, and KU49 strains were confirmed to contain plasmid. The isolated Ti plasmids were digested with 4 kinds of restriction enzymes, respectively. According to the result of the cleavage patterns, we confirmed that these plasmids in the KU12, KU13, KU14 and KU49 strains are different.
Keywords