고려인삼중 지용성 성분이 인체암 세포의 수종 효소활성에 미치는 영향.

Effects of Petroleum Ether Extract of Ginseng Root on Some Enzyme Activity in Human Colon Cancer Cells

본 연구는 인삼중 지용성 성분이 인체암 세포의 증식억제와 암세포내 효소 활성에 미치는 영향을 구명하고자 실시하였다. 인체 장암 세포인 HRT-18, HCT-48 및 HT-29등을 대상으로 인삼추출물 처리시 각 암세포의 증식율과 암세포내 효소 즉, sucrase, lactase, maltase 및 trehalase등 disaccharidas 활성을 측정한 바 다음과 같은 결과를 얻었다. 1. HTR-18, HT-29 및 HCT-48의 doubling time은 각각 약 20, 22, 24시간이 및 되었다. 2. 각 암세포의 증식율은 배양액 중 CX 함량의 증가와 연장에 따라 점차 더 억제되었다. 3. 인삼 extract를 함유하는 배양액에서 배양된 HRT-18 및 HCT-48 암세포의 sucarse활성은 각각 362% 및 577% 증가하였고, lactase(317%, 334%), maltase(134% 및 153%) 및 trehalase(311% 및 203%) 활성도 다 같이 유의성있게 증가하였다.

This study was devised to observed the growth inhibition and change of disaccharidase activities of human colon cancer cells cultured in medium containing the ginseng extract. Three species of human colon cancer cell lines, HRT-18, HCT-48 and HT-29, were used for the experiment. The activities of sucrease, lactase, maltase and trehalase in the cancer cells were determined. The results obtained are summarized as follows; 1. The doubling times of the HRT-18, HT-29 and HCT-48 were about 20,22 and 24 hours, respectively. 2. The growth rates of the HRT-18 and HCT-48 in culture medium containing the ginseng extract were inhibited gradually according to increase of the concentration of ginseng extract and extension of the incubation time. 3. The activities of disaccharidase in HRT-18 and HCT-48 cultured in the medium containing the ginseng extract were increased compared with control group as follows;