Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Dissimilatory Nitrate Reduction Characteristics of Indigenous Soybean Rhizobia Distributed in Korea Soils

한국에 분포되어 있는 토착대두 근류균의 질산 환원 특성

  • Choi, Young-Ju (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
  • Choi, Yong-Lark (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
  • Yun, Han-Dae (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
  • Ryu, Jin-Chang (Institute of Agricultural Science) ;
  • Lee, Sang-Kyu (Institute of Agricultural Science) ;
  • Cho, Moo-Je (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University)
  • 최영주 (경상대학교 농과대학 농화학과) ;
  • 최용락 (경상대학교 농과대학 농화학과) ;
  • 윤한대 (경상대학교 농과대학 농화학과) ;
  • 유진창 (농촌진흥청 농업기술연구소) ;
  • 이상규 (농촌진흥청 농업기술연구소) ;
  • 조무제 (경상대학교 농과대학 농화학과)
  • Published : 1986.06.30
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Abstract

Eightyseven strains of indigenous Rhizobia were isolated from the nodule of soybean cultivar, Danyup, cultivated in four different soils sampled from continuously soybean cultivated and newly reclaimed fields. The strains were grouped into Bradyrhizobium japanicum (slow-grower:55 strains) and Rhizobium fredii (fast-grower: 32 strains). The both groups could be divided into two sub-groups according to the denitrification characteristics, that is, denitrifying fast-grower (F-I), nitrate respiring fast-grower (F-II), denitrifying slow-grower (S-I). and nitrate respiring slow-grower (S-II). Among the 87 isolates, F-I, F-II, S-I and S-II sub-groups were 10, 22, 48 and 7 strains, respectively. The one-and two-dimensional polyacrylamide gel electrophoretic pattern of the four sub-groups were compared and discernible difference was observed between fast and slow-grower, but the difference was not discernible between subgroups within the same growth rate group.

연속적으로 대두를 재배한 토양과 신개간지 토양에서 재배한 대두 품종 단엽의 근류로부터 토착대두 근류근 87종을 분리하고 이들을 생장속도에 따라 Bradyrhizobium japonicum (slow-grower) 및 Rhizobium fredii (fast-grower)로 분류한 결과 전자가 55종, 후자가 32종이었다. 이두그룹의 대두 근류균을 질산염 환원특성에 따라 denitrifying fast-grower(F-1), nitrate respiring fast-grower(F-II), denitrifying slow-grower(S-I), 및 nitrate respiring slow-grower (S-II)로 세분 되었으며, 분리균주 총 87종중 S-I, S-II, F-I 및 F-II의 수는 각각 10, 22, 48 및 7종이었다. 이들 각 group 대두 근류균의 균체단백질의 전기영동 pattern상의의 차이를 1차 및 2차원 전기영동으로 분석한 결과 slow 및 fast-grower간에는 현저한 차이를 관찰할 수 있었으나 동일 생장속도를 가진 것 중 denitrifer와 nitrate repirer간에는 큰 차이가 없었다.

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