Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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An Extraction of Barley Protein and a Comparison of the Protein Composition of Some Barleys -Electrophoretic Pattern of Barley Protein-

보리단백질의 추출 및 품종간 조성비교 -II. 보리단백질의 품종간 조성비교-

  • Kim, Ze-Uook (Department of Food Science and Technology, College of Agriculture Seoul National University) ;
  • Kim, Jung-Sang (Department of Food Science and Technology, College of Agriculture Seoul National University)
  • 김재욱 (서울대학교 농과대학 식품공학과) ;
  • 김정상 (서울대학교 농과대학 식품공학과)
  • Published : 1986.03.30
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Abstract

Total protein from 4 barley varieties(Olbori, Young San-bori, Sacheon 6, and Suwon 228) was separated into albumin, globulin hordein and glutelin fractions by the Osborne method and tile modified Osborne method in a previous report. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for the protein fractions revealed that there was a little difference in the polypeptide composition of each fraction among four varieties. The comparision of hordeins and hordein-Is by polyacrylamide gel electrophoresis at pH 3.0 showed a marked difference among the varieties. Hordein-I contained a high level of glutamic acid and proline and low level of lysine. And (here was little difference in amino acid composition of hordein-Is which were extracted from the 4 varieties.

네품종의 보리(올보리, 영산보리, 사천 6호, 수원 228호)로 부터 Osborne의 방법과 이것을 변형한 전보의 방법으로 단백질을 분획하여 albumin, globulin, hordein 및 glutelin 획분을 얻어 이들 각획분들을 SDS-PAGE로 분석한 결과 품종간 polypeptide 조성 차이를 관찰할 수 있었으며 특히 hordein과 hordein-I을 pH 3에서 PAGE를 수행 하였을 때 각 품종사이의 pattern에 명확한 차이를 볼 수 있었다. 한편, hordein-I의 아미노산조성은 glutamic acid와 proline 함량이 높았으나 품종간의 아미노산조성차이는 거의 볼 수 없었다.

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