한국균학회지 (The Korean Journal of Mycology)
- 제14권4호
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- Pages.273-280
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- 1986
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Autolytic Enzyme을 이용한 Rhizopus oryzae의 원형질체 생성과 그 특성에 관한 연구
Characteristics of Protoplast Induction by Autolytic Enzyme of Rhizopus oryzae
- Rhee, Young-Ha (Department of Microbiology, College of Natural Sciences, Chungnam National University) ;
- Choi, Yong-Keel (Department of Biology, College of Natural Sciences, Hanyang University)
- 발행 : 1986.12.30
초록
Rhizorus oryzae의 생장 및 autolysis에 따른 여러가지 autolytic enzymes의 활성도 변화와 이 autolytic enzyme system을 이용한 원형질체 생성에 관하여 조사하였다. Autolytic·phase의 culture filtrate 내에 함유된 autolytic enzyme은 Rh. oryzae 균사체로부터의 원형질체 생성에 효율적인 세포벽 분해효소로 작용하였으며 Autolytic enzyme중 원형질체 생성은 proteolytic 및 chitosanase activity와 가장 긴밀하게 관련되어 있었다. 이와같은 autolytic enzyme을 이용한 원형질체 생성은 10시간 배양한 균사체에 0.5M mannitol을 사용하였을 때 최고의 수율을 보였으며 원형질 생성의 최적온도 및 pH는 각각
The degree of autolysis and lytic enzyme production in the culture filtrate of Rhizopus oryzae was investigated. The formation of protoplast by using autolytic enzymes from Rh. oryzae was also attempted. Protoplasts were liberated from Rh. oryzae mycelium by lytic enzymes present in autolytic-phase culture filtrate. Maximum release of chitosanase and proteolytic enzyme into culture filtrate during autolysis was corresponded to maximum protoplast-liberating activity. High yields of protoplasts were obtained from 10 hr-age of Rh. oryzae mycelium with 0.5 M mannitol as osmotic stabilizer. The optimum temperature and pH for mycelium digestion were