The Korean Journal of Mycology (한국균학회지)
- Volume 14 Issue 2
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- Pages.101-107
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- 1986
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Studies on the Characterization of Carboxyl Proteinase in Poria cocos
복령의 Carboxyl Proteinase의 분리 정제 및 그 성질에 관한 연구(II)
- Min, Tae-Jin (Department of Chemistry, Dongguk University) ;
- Park, Sang-Shin (Department of Chemistry, Dongguk University) ;
- Moon, Soon-Ku (Department of Chemistry, Dongguk University)
- Published : 1986.06.30
Abstract
The properties of carboxyl proteinase which was contained in Poria cocos (Schw.) Wolf were investigated by means of the purification with 0.65 ammonium sulfate saturation, DEAE cellulose and Sephadex G-75 gel filtration. This enzyme was found to hydrolyze only peptide bond between glutamyl-L-tyrosine of carbobenzoxy-L-glutamyl-L-tyrosine among the synthetic substrates of carbobenzoxy-L-glutamyl-L-tyrosine, hippuryl- L-phenylalanine and hippuryl-L-arginine. This enzyme was inhibited by