Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Acridine Orange-induced Changes in Cell Wall of Salmonella pullorum

Acridine Orange에 의한 Salmonella pullorum의 세포벽 변화

  • Kim, Jong-bae (College of Veterinary Medicine, Seoul National University) ;
  • Mah, Jum-sool (College of Veterinary Medicine, Seoul National University)
  • 김종배 (서울대학교 수의과대학) ;
  • 마점술 (서울대학교 수의과대학)
  • Received : 1985.09.01
  • Published : 1985.10.20
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Abstract

Salmonella pullorum strain W was serially passaged on the brain heart infusion agar containing acrdine orange(AO) as a concentration of 100 mcg/ml. S. pullorum AO60 and S. pullorum AO150, which were subcultured 60 and 150 passages on AO media, were examined for permeability barrier function of the cell wall. AO60 and AO150 were appeared to be decreased in susceptibility against hydrophobic substances such as crystal violet, chloramphenicol and rifamycin, which might be resulted from the changes of permeability barrier function of the cell wall. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial protein, the protein profiles of AO6O and AO150 didn't differ significantly from W, but increased amount of the band of MW 140,000-145,000 was confirmed. And [G+C] contents of DNA in AO60 and A0150 were decreased than that of W.

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