A Micro-quantification of Abscisic Acid from Plant Tissue by Pentafluorobenzyl Esterification Using GLC

Pentafluorobenzyl ester 화(化)에 의(依)한 미량(微量) Abscisic Acid 의 GLC 분석법(分析法)

  • Jeong, Young-Ho (Agricultural Chemicals Research Institute, Rural Development Administration) ;
  • Hong, Moo-Ki (Agricultural Chemicals Research Institute, Rural Development Administration) ;
  • Song, Byung-Hun (Agricultural Chemicals Research Institute, Rural Development Administration)
  • 정영호 (농촌진흥청 농약연구소) ;
  • 홍무기 (농촌진흥청 농약연구소) ;
  • 송병훈 (농촌진흥청 농약연구소)
  • Published : 1985.12.30

Abstract

A new method for micro-quantification of abscisic acid from plant tissue is described. GLC-ECD method of abscisic acid pertafluorobenzyl ester synthesized by reaction of plant extract with pentafluorobenzyl bromide showed higher sensitivity than that of abscisic acid methyl ester, and the detection limit of abscisic acid by the described method was as low as 5 pg.

식물체내(植物體內) ABA의 미량분석법(微量分析法) 개발(開發)을 위(爲)하여 시험(試驗)한 결과(結果) 식물체(植物體)의 ABA 추출물(抽出物)을 pentafluorobenzyl bromide로 ester 화(化)시켜 ABA-PFB ester 형태(形態)로 GLC-ECD 분석(分析)하므로서 ABA-methyl ester 보다 높은 감도(感度)를 보여 5 pg의 ABA 까지 측정(測定)이 가능(可能)하였다.

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