Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Effect of Ethidium Bromide on Anomalous Absorbance-Temperature Profile, of Calf Thymus DNA, induced by Spermine

송아지 胸線 DNA의 Spermine에 依하여 誘發되는 異例的 吸光度-溫度 樣相에 미치는 Ethidium Bromide의 影響

  • Chan Yong Lee (Department of Chemistry, College of Sciences, Chungnam Natonal University) ;
  • Thong Sung Ko (Department of Chemistry, College of Sciences, Chungnam Natonal University)
  • 이찬용 (忠南大學校 理科大學 化學科) ;
  • 고동성 (忠南大學校 理科大學 化學科)
  • Published : 1985.06.20
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Abstract

An anomalous absorbance-temperature profile of calf thymus DNA, having a trough preceding the initiation of the melting, occurs at the spermine concentration, where the DNA collapses into a compact structure. The cooperativity, enthalpy, and the midpoint of the phase transition to the trough region are more sensitive to ethidium bromide than those of the Tm region. As the concentration of ethidium bromide added is increased, the peak size of the trough is decreased, while the Tm remains essentially constant.

송아지 胸線 DNA가 單分子的 凝縮된(collapsed) 構造를 이루는 spermine 濃度에서 얻어지는 異例的 吸光度-溫度 樣相(anomalous absorbance-temperature profile)의 trough 領域에 이르는 相變移에 對한 cooperativity, enthalpy, 및 相變移 midpoint를 구하여 그 값들을 Tm 領域의 값들과 比較한 結果 그 두 그룹의 값들은 서로 相異하였으며 前者의 값들이 後者의 값들 보다 ethidium bromide에 對하여 더 銳敏하였다. Ethidium bromide 濃度를 增加시킴에 따라서 그 trough의 깊이가 작아져서 終局的으로 없어졌으나 그와 對照的으로 Tm은 實驗的으로 一定하게 維持되었다. 本 硏究結果를 基礎로 하여 DNA 凝縮 메카니즘을 推理하였다.

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