Journal of Plant Biology
- Volume 28 Issue 3
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- Pages.233-241
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- 1985
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- 1226-9239(pISSN)
Isolation and Culture of Protoplasts from Hypocotyl-derived Callus of Soybean (Glycine max)
대두 (Glycine max) 부배유 유래 칼루스의 원형질체 분리 및 배양
Abstract
The isolation and culture of protoplasts from hypocotyl-derived calluses of Glycine max (L.) Merr. cv. Jangyeop were obtained by digestion for 6 hrs in an enzyme solution containing 3.5% cellulase, 1.5% macerozyme, 10% sorbitol and 0.1% CaCl2.2H2O at pH 5.8. Newly formed cell wall of protoplasts cultured in MS agar medium containing 10
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