미생물학회지 (Korean Journal of Microbiology)
- 제23권1호
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- Pages.25-33
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- 1985
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Cellulomonas가 생산하는 균체의 Cellulase의 분리 및 분리된 효소의 작용기작
Fractionation of Extracellular Cellulase Pproduced by Cellulomonas and Reaction Mechanisms of the Isolated Enzymes
- Kim Byung Hong (Department of Microbiology, University College, Newport Road) ;
- Wimpenny, J.W.T. (Department of Microbiology, University College, Newport Road)
- 발행 : 1985.03.01
초록
Cellulomonas flavigena KIST 321이 생산하는 균체외 cellulose 분해효소를 gel filtration법과 ion-exchange chromatography법으로 3종의 다른 효소를 분리하여 이들을 A, B, 및 C 효소로 명명하였다. 분리된 각 효소를 결정적 기질에 처리하여 일어나는 기질의 구조변화를 적회전 분광법과 X-ray crystallography법으로 측정하여 다음 결론을 얻었다. B효소는 결정성 cellulosem이 구성단위인 glucopyranose의 불안정화로 그 결정도를 감소시키는
The cell-free cellulolytic enzyme was separated into 3 different enzyme proteins by gel-filtration and ion-exchange chromatography. These fractions were named enzyme A, enzyme B and enzyme C. The mode of action of each of the separated enzymes on crystalline cellulose was examined using infrared spectroscopy and X-ray crystallography. It was concluded that enzyme B is of the
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