Determination of Chloramphenicol in Milk by High Performance Liquid Chromatography

HPLC를 이용한 우유중의 클로람페니콜의 정량 분석

  • 김경례 (성균관대학교 약학대학) ;
  • 김정한 (연세대학교 식품공학과) ;
  • 최경숙 (연세대학교 식품공학과)
  • Published : 1985.02.01

Abstract

Seven different sorbents were evaluated for their adsorptivity and desorptivity of antibiotic, chloramphenicol. Among the sorbents studied, Carbopak B was found to be the most efficient in enriching the chloramphenicol from dilute aqueous solution. Interfering components in the milk matrix could be washed off by water and petroleum ether from Carbopak B column, while the chloramphenicol was retained on the surface of Carbopak B. The method of simple and efficient purification and enrichment of chloramphenicol using Carbopak B, followed by quantitative analysis employing $C_{18}$ reversed phase high performance liquid chromatography has been applied to the determination of chloramphenicol in milk.

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