Applied Biological Chemistry
- 제28권2호
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- Pages.92-97
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- 1985
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
자외선 조사(照射)에 의한 Agrobacterium tumefaciens Arginine 요구주(要求株)의 유도와 선발
Mutation and Selection of Agrobacterium tumefaciens Arginine Auxotroph by UV Irradiation
- Lee, Yearn (Department of Agric. Chem., College of Agriculture, Chonnam National University) ;
- Park, Ro-Dong (Department of Agric. Chem., College of Agriculture, Chonnam National University) ;
- Kim, Kwang-Sik (Department of Agric. Chem., College of Agriculture, Chonnam National University)
- 발행 : 1985.06.30
초록
Agrobacterium tumefaciens A 136에 자외선은 조사(照射)하여 Ti-plasmid 숙주로 이용할 수 있는 arginine 영양요구주를 선발하고자 하였다. A. tumefaciens의 생육을 600nm에서의 흡광도(吸光度)로 측정할 때 TY배지에서 2% 접종시 7시간 부터 16시간 사이에 대수기를 보였고 generation time은 4.8시간이었다.
UV irradiation method was applied to Agrobacterium tumefaciens A 136 to obtain arginine auxotrophic mutant which is applicable as a host of Ti-plasmid. When the bacterial growth was measured at 600 nm, it showed the exponential phase between 7 and 16 hours after 2% inoculation (v/v) in TY medium and the generation time of 4.8 hours. Survival rate of
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