Effect of Dietary Fibers on Serum and Liver Lipids of Cholesterol-fed Rats

식이성(食餌性) 섬유소(纖維素)가 콜레스테롤 식이(食餌) 흰쥐의 혈청(血淸) 및 간장지질(肝臟脂質)에 미치는 영향(影響)

This experiment was designed to investigate the effect of dietary fibers on serum and liver lipids of cholesterol-fed rats. Forty-two male rats of Sprague-Dawley strain weighed $145{\pm}10\;g$ were divided into 6 groups, each group receiving a different diet for 6 weeks, i.e., basal diet, basal diet plus 0.5% cholesterol without fiber, basal diet plus 0.5% cholesterol and 5% pectin, basal diet plus 0.5% cholesterol and 5% agar, basal diet plus 0.5% cholesterol and 5% pectin plus tannic acid mixture and basal diet plus 0.5% cholesterol and 5% tannic acid. The lowest net weight gain and digestibility were found in 5% tannic acid-containing group. The weight of kidney, heart and lung was significant by different, however, those of liver and spleen was not significantly different among the groups tested. GOT and GPT of serum were significantly higher in 0.5% cholesterol-containing group without fiber, whereas those of 5% pectin-containing group were significantly lower. Highest total serum protein content was found in 0.5% cholesterol-containing group without fiber. However, albumin and A/G ratio were not significant. The content of total lipid and cholesterol in serum were not significant by different among the groups studied, whereas crude lipid contents of liver in 5% tannic acid and pectin plus tannic acid-containing groups were significantly lower. Cholesterol content in the liver was significantly lower in 5% tannic acid-containing group. Crude lipid and sterol content of feces were significantly higher in 5% pectin-containing group.

최근(最近) 식이성(食餌性) 섬유소(纖維素)가 인간(人間)의 질병발생(疾病發生)에 예방효과(豫防效果)가 있음이 밝혀진 바, 특히 동맥경화증(動脈硬化症)에 관련(關聯)한 cholesterol 저하효과(低下效果)를 조사(調査)하기 위하여 본(本) 실험(實驗)에서는 pectin, agar, pectin+tannic acid, tannic acid 각(各) 5%를 0.5% cholesterol 식이(食餌)에 첨가(添加)하여 Sprague-Dawley계(系) 흰쥐(male)에게 6 주간(週間) 급여(給與)하였다. 이에 흰쥐의 체중증가량(體重增加量), 소화흡수율(消化吸收率), 각종(各種) 장기(臟器) 중량(重量), 혈지(血漬)과 간장(肝臟) 및 분변(糞便)중의 지질(脂質) cholesterol함량(含量)을 측정(測定)한 결과(結果)는 다음과 같다. 1. 체중증가량(體重增加量)과 소화흡수율(消化吸收率)은 5% tannic acid 첨가군(添加群)만이 유의(有意)하게 낮았다. 2. 각종(各種) 장기(臟器)의 중량(重量)에서 간장(肝臟) 비장(脾臟)은 유의성(有意性)이 없었으며 신장(腎臟)은 PEC, AA군(群)에서 감소(減少)하였고, 심장(心臟)은 basal군(群), 폐장(肺臟)은 basal+chol 군(群)에서 유의(有意)하게 증가(增加) 하였다. 3. 혈청(血淸)중 GOT, GPT는 0.5% cholesterol 첨가군(添加群) (basal+chol.)만이 현저하게 높았으며 PEC 군(群)에서 유의(有意)하게 감소(減少)하였다. Total protein은 basal+chol. 만이 유의(有意)하게 높았으나 albumin, A/G ratio는 유의성(有意性)이 없었다. 4. 혈청(血淸)중 tolal lipid와 cholesterol 함량(含量)에는 유의성(有意性)이 나타나지 않은 반면, 간장(肝臟)중 조지질(粗脂質) 및 sterol 함량(含量)은 PEC 군(群)만이 유의(有意)하게 증가(增加)하였다.