한국인삼의 연근별 단백질 및 아미노산 조성

Proteins and Amino Acid Composition of Korea Ginseng Classified by Years

연근별로 인삼 단백질의 생화학적 특성을 체계적으로 규명하기 위하여 polyacrylamide gel 전기영동, SDS-PAGE, gel filteration 및 아미노산 자동분석기에 의한 실험결과는 다음과 같다. 인삼의 수용성 단백질을 Sephadex G-200으로 분획하여 얻은 2개의 peak 중주 단백질은 SDS-polyacrylamide gel 전기영동에 의해 분자량은 43,000으로 추정되었다. Polyacrylamide gel 전기영동 및 SDS-PAGE에 의한 band의 수는 연근에 따라 별 차이 없이 각각 8 및 $7{\sim}11$개 확인되었다. 인삼의 수용성 단백질과 그의 주 단백질을 아미노산 분석한 결과 arginine이 각각 45.17과 57.36%로 최대의 함량을 나타내었고 methionine과 cystine의 함량은 매우 낮았다.

For the systematic investigation of biochemical characteristics of Korean ginseng protein by years, protein fractions were analyzed by the techniques of polyacrylamide gel electrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration, while the amino acid composition was studied by amino acid autoanalyzer. Results of polyacrylamide gel electrophoresis and SDS-PAGE showed a few difference in pattern and number of bands depending on the age of the root. However, the number of bands obtained from polyacrylamide gel electrophoresis and SDS-PAGE was 8 and 7 to 11, respectively. When water extracted proteins were fractionated by Sephadex G-200, the main peak among 2 peaks was collected and lyophillized. Its mol. wt. was extimated to be 43,000 by the SDS-PAGE method. In amino acid composition of water extracted protein and main fraction of gel filtration, arginine content was the highest, 47.17% in water extracted protein and 57.36% in main fraction followed by glutamic acid and asparatic acid. On the contrary, cystine and methionine contents were very low in both cases.