Separation and Determination of Saikosaponins in Bupleuri Radix with HPLC

HPLC에 의한 시호(柴胡) Saponin의 분리 및 정량

  • Han, Dae-Suk (College of Pharmacy, Seoul National University) ;
  • Lee, Dug-Keun (College of Pharmacy, Seoul National University)
  • Published : 1985.09.30

Abstract

The optimal condition for the determination of saikosaponin a and d, the major pharmacologically active saponins of the roots of Bupleurum falcatum, was studied with the conversion of these saponins into diene saponins $(saikosaponin\;b_1\;and\;b_2)$. The complete separation and quantitative analysis of these saponins were performed by the method of high performance liquid chromatography using $NH_2$ column. The conversion of saikosaponin a and d into diene saponins under gastric pH was calculated. Thirty-three percent of saikosaponin a was converted to saikosaponin $b_1$ and 63 percent of saikosaponin d was converted to saikosaponin $b_2$.

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