비름과식물즙액에 의한 담배 모자이크 바이러스의 감염억제효과

Inhibition of Tobacco Mosaic Virus Infection by the Crude Sap Extracted from Amaranthaceae Plants

비름과 식물즙액에 의한 TMV 감염억제효과는 공시한 6종의 비름식물중 비름이 $94.6\%$로 가장 높은 효과를 나타냈다. 비름즙액의 물리화학적성질중, 내열성은 $60^{\circ}C$, 내보존성은 1일, 내희석성은 1/4 희석농도까지 높은 효과를 나타냈다. 한편 수소이온 농도의 변화에는 매우 안정된 성질을 보였으며, 특히 산성영역에서는 모두 $90\%$ 이상의 억제효과를 보였다. 그러나 비름즙액을 활성탄으로 처리하였을 경우는 TMV에 대한 억제효과는 거의 소실되었다. 비름즙액에서 추출한 단백질은 전기영동결과, 3종의 주성분으로 분리되었고 이들 중 top 성분에서 TMV 억제효과가 나타났다. 비름즙액의 실용화를 위한 도말액 효과특속성은 2회 관수에서 $80\%$ 정도의 억제능을 보였다.

Crude sap, which was extracted from six Amaranthaceae plants, inhibited local lesion formation on Nicotiana glutinosa by tobacco mosaic virus(TMV) infection. Especially the remark. able inhibitory effect to TMV infection was shown on leaves of N. glutinosa precoated with the sap from Amaranthus mangostanus. The inhibitory activity of the sap from A. mangostanus was stable to storage in vitro for I day and to dilution 1/4 of the sap with distilled water. However, its activity was lost when the sap was heated at $70^{\circ}C\;to\;100^{\circ}C$ for 10 minutes. When the leaves of N. glutinosa precoated with the sap were sprayed with water, the inhibitory effect to TMV infection was maintained for 2 days. The A. mangostanus sap readjusted pH 3, pH 5, or pH 9 with 1 N HCl or 1 N NaOH did not decline the inhibitory action but the sap absorbed with $5\%\;to\;15\%$ charcoal completely lost their action. The protein components purified from A. mangostanus sap revealed three major bands by $5\%\;to\;15\%$ polyacrylamide gel electrophoresis and the top component of which showed the inhibitory action to TMV infection.