Korean Journal of Veterinary Research (대한수의학회지)
- Volume 24 Issue 1
- /
- Pages.40-49
- /
- 1984
- /
- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
Studies on the Factors Influencing the Transformation in Escherichia with pBR322 DNA
Escherichia coli의 pBR322 DNA 형질전환에 관여하는 인자에 관한 연구
- Yoo, Han-sang (College of Veterinary Medicine, Seoul National University) ;
- Mah, Jum-sool (College of Veterinary Medicine, Seoul National University)
- Received : 1984.03.05
- Published : 1984.06.01
Abstract
To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM
Keywords