Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Electron Microscopic Study of Protoplast Formation from the Conidiospore of Trichoderma koningii

Trichoderma koningii의 conidiospore로부터의 원형질체 생성에 관한 전자현미경적 연구

  • Park, H.M. (Dept. of Microbiol. Seoul National University) ;
  • Lim, H.M. (E.M. Lab., College of Med., Yon Sei University) ;
  • Hong, S.W. (Dept. of Microbiol. Seoul National University) ;
  • Hah, Y.C. (Dept. of Microbiol. Seoul National University)
  • 박희문 (서울대학교 자연대 미생물학과) ;
  • 임헌만 (연세대학교 의과대학 전자현미경실) ;
  • 홍순우 (서울대학교 자연대 미생물학과) ;
  • 하영칠 (서울대학교 자연대 미생물학과)
  • Published : 1984.12.01
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Abstract

Fine structure of dormant and swollen conidiospore from Trichoderma koningii and the mechanism of protoplasting from the conidiospore were studied by scanning and transmission electron microscopy. The cell wall of dormant conidiospore was two-layered structure which consisted of electron dense outer layer and electron transparent inner layer. After 8.5 hrs incubation. the conidiospore was swollen and the outer layer of cell wall shown unequal thickness and partial breakage. Protoplast was released through the pore which has been formed by the breakage of outer layer and dissolution of newly synthesized cell wall for germ-tube formation. Swollen conidiospore and protoplast in releasing process contained various cell organelles and vacuoles with electron dense materials. The protoplast contained looser cytoplasm and had no cell wall materials outside of plasmamembrane.

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