사람 태반혈청내의 항HLA항체 정제

Purification of Anti-HLA Antibodies in Human Placenta Sera

  • 임병욱 (가톨릭의과대학 미생물학교실) ;
  • 한훈 (가톨릭의과대학 미생물학교실) ;
  • 유문간 (가톨릭의과대학 미생물학교실) ;
  • 김태규 (가톨릭의과대학 미생물학교실) ;
  • 김금용 (가톨릭의과대학 미생물학교실) ;
  • 이종훈 (가톨릭의과대학 미생물학교실)
  • Lim, Byung-Uk (Department of Microbiology, Catholic Medical College) ;
  • Han, Hoon (Department of Microbiology, Catholic Medical College) ;
  • Rhyu, Moon-Gan (Department of Microbiology, Catholic Medical College) ;
  • Kim, Tae-Kyu (Department of Microbiology, Catholic Medical College) ;
  • Kim, Gum-Ryong (Department of Microbiology, Catholic Medical College) ;
  • Lee, Chong-Hoon (Department of Microbiology, Catholic Medical College)
  • 발행 : 1984.12.31

초록

To determine the existence of anti-HLA antibodies finally in 220 human placental extracts to be proved negative antiserum by previous anti-HLA A,B,C antibody screening procedure, the present study was performed by fractionation of immunoglobulins using saturated ammonium sulfate and by simple batch method on DEAE cellulose. Thereafter using known 150 T-lymphocyte panels, complement-dependent microlymphocytotoxicity test was performed to observe the existence of anti-HLA antibodies and the degree of the antibody response of the concentrates. The following results were obtained: 1. Of total 141 placental sera concentrated 45 cases(31.9%) were showed significant anti-HLA A,B,C antibody response after concentration(Excellent, 19(13.5%), Good, 3(2.1%), Weak, 23(16.3%)). 2. Anti-HLA specificities of placental sera obtained after concentration were A2, A24, B13, B27, B44, B51, CN1, C7. 3. A new type C new-1 anti-HLA antibody that is only expressed in Korean people, was obtained. 4. 79 placental sera purrified by simple batch method using DEAE cellulose were showed negative anti-HLA antibody responses.

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