Bulletin of the Korean Chemical Society

  • 제5권6호
  • /
  • Pages.253-259
  • /
  • 1984
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Microprocessor Based Laser Induced Fluorometry I. Development of System and Application to Liquid Chromatography

  • Kim, Ha-Suck (Department of Chemistry, Seoul National University) ;
  • Park, Chan-Seung (Department of Chemistry, Seoul National University) ;
  • Hwang, Eui-Jin (Department of Chemistry, Seoul National University) ;
  • Chol, Q.-Won (Department of Chemistry, Seoul National University)
  • 발행 : 1984.12.20
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초록

An analytical applicability of the fluorescence detection with an optical multichannel analyzer to organic dyes was studied in this work. Continuous acquisition of spectra was possible with the use of a microcomputer. The maximum acquisition rate of a spetrum with 70-point average was about 3 seconds. Floppy discs were used to store data for later use in processing. Laser induced fluorescence detector in High Performance Liquid Chromatography was chosen for an application. Fluorescein below $10^{-15}g$ was detected satisfactorily using this system. With the help of microcomputer, three dimensional chromatograms of time-wavelength-intensity were obtained.

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