Cell Biological Studies on the Mechanism of Development and Differentiation Ⅷ 2. Effects of Peptide on cAMP Level in Corn Endosperm

생체발생 및 문화기구의 세포생물학적 연구 Ⅷ 2. 옥수수 배젖에서 Peptide가 cAMP Level에 미치는 영향

  • Published : 1984.03.01

Abstract

Activities of corn endosperm adenylate cyclase and phosphodiesterase were found right after germination, and phosphodiesterase activity was shown to increase steadily. Protease activity was also found. Corn peptide fraction purified by using Sephadex G-25 column was shown to enhance corn phosphodiesterase activity buy inhibit bovine phosphodiesterase activity. And the fraction inhibits corn adenylate cyclase activity. Trypsintreated peptide fraction was shown to enhance phosphodiesterase activity 80% compared to that of native peptide fraction. However, in case of DNase phosphodiesterase was shown to be innocuous. According to cumulative results, it is more likely that peptide fraction produced by protease inhibits adenylate cyclase activity and enhance phosphodiesterase, decreasing cAMP level.

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