Isolation of Aspergillus fumigatus and Properties of It's Enzyme for Rhodotorula glutinis Cell Wall Lysis

Rhodotorula glutints 세포벽 용해효소를 생산하는 Aspergillus fumigatus의 분리와 그 효소의 특성

A fungus producing cell wall lytic enzyme for Rhodotorula glutinis was isolated from local soil and identified partially as a species of Aspergillus fumigatus group. Thd cell wall lytic enzyme was an inducible exoenzyme and composed of at least lytic polysaccharidase and protease which act cooperatively in the lysis of intact cells. The lytic polysaccharidase was not able to hydrolyze ${\beta}-1,\;3\;and\;{\beta}-1$, 6-glucan which have the same types of bond as found in the cell wall of Ascomycetous yeasts. The lytic polysaccharidase alone was sufficient to hydrolyze the fractionated cell wall (alkali-insoluble residues) of R. glutinis, whereas it showed low activity against intact cells.

Rhodotorula gl$\varkappa$tinis 세포벽에 작용하는 용해 효소 생산곰팡이를 토양으로부터 분리하였고, Aspergillus f'||'&'||'micro;mig$\alpha$tus에 속하는 species로 동정되었다. 이 세포벽 용해효소는 세표외 유도효소였으며 lytic polysaccharidase 와 protease로 구성되어 생세포 용해에 공동으로 착용하였다. 이 lytic polysaccharidase는 Ascomycetous 효모에서의 주 구성 결합인 ${\beta}-1,3-$와 ${\beta}-1$, 6-glucan에는 작용치 않았다. 이 효소는 생세포에는 역가가 낮았지만 R. glutinis의 분획된 세포액에는 protease의 도움없이 작용할 수 있었다.