Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Exo-maltotetraohydrolase of Pseudomonas stutzeri IAM 12097 -Part I. Purification of Exo-maltotetraohydrolase-

Pseudomonas stutzeri IAM 12097의 Exo-maltotetraohydrolase에 관한 연구(硏究) -제일보(第一報). Exo-maltotetraohydrolase의 정제(精製)-

  • Lee, Mi-Ja (Department of Food Science and Technology, College of Agriculture, Chungbuk National University) ;
  • Chung, Man-Jae (Department of Food Science and Technology, College of Agriculture, Chungbuk National University)
  • 이미자 (충북대학교 식품가공학과) ;
  • 정만재 (충북대학교 식품가공학과)
  • Published : 1984.06.30
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Abstract

The optimum culture time and initial pH, for the production of exo-maltotetraohydrolase from Pseudomonas stutzeri IAM 12097, in the trypticase medium were 36 hrs and pH 6.3, respectively. Exo-maltotetraohydrolase was purified by $(NH_4)_{2}SO_4$ and two times of column chromatography on DEAE-cellulose. Specific activity of the purified enzyme was 108.6U/mg protein and yield of the enzyme activity was 9.4%. The purified enzyme showed a single band on polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis.

Pseudomonas stutzeri IAM 12097의 trypticase 배지(培地)에서 36시간(時間), initial pH는 6.3일때 Exo-maltotetraohydrolase가 최대로 생산되었다. Exo-maltotetraohydrolas, 황산(黃酸)암모니아분획(分劃)과 2회(回)의 DEAE-cellulose column chromatography에 의하여 정제(精製)하였으며 정제효소(精製酵素)의 specific activity는 108.6 u/mg protein, 수율(收率)은 9.4%이었다. 본정제효소(本精製酵素)는 polyacrylamide gel electrophoresis와 SDS-polyacrylamide gel electrophoresis에 의하여 각각(各各) 단일(單一) band를 나타내었다.

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