Nutritional and Biochemical Studies on the Pollen toads -1. Studies on Lipid Compositions of Sunflower Pollen toad and Effects of Its Pollen toad on Liver Cholesterol Metabolism in Mouse-

화분립(花粉粒)의 영양생화학적(營養生化學的) 연구(硏究) -1. 해바라기 화분립(花粉粒)의 지질조성(脂質組成)과 Mouse 간장(肝臟) 콜레스테롤 대사(代謝)에 미치는 영향(影響)-

For the purpose of investigating whether the administration of sunflower pollen load has any influence upon liver cholesterol metabolism in mouse, lipids were isolated from sunflower pollen load, identified and quantitated by thin-layer and gas liquid chromatographies. We also studied changes in liver cholesterol level in mouse according to the amount and the period of pollen load administration. Lipids of sunflower pollen load were constituted 84.10f of neutral lipid, 10.50% of glycolipid and 5.40% of phospholipid. The main fatty acid contents of neutral lipid, glycolipid and phospholipid were ranged 28.48 to 33.70% of linoleic acid, 12.90 to 47.50% of palmitic acid ana 11.20 to 12.20% of oleic acid, however, phospholipid contained more palmitic acid than the other lipids. The body weight of the Pollen fed mouse significantly increased during experimental Period in comparison with control group. From the fact tat the ratio of liver weight to body weight of pollen fed mouse was smaller than that of control group, it was proved that liver lipid metabolism of pollen fed mouse was more active than that of control group. During early experimental period, liver cholesterol level had been increased according to pollen load administration(P.O), and then the level decreased rapidly to the similar level to that of control group at the end of the period.

해바라기 화분립이 mouse의 간장에서 cholesterol 대사에 어떤 영향을 미치는가를 조사하기 위해서 해바라기 화분립으로부터 추출한 지질을 column chromatography, thin-layer chromatography 및 gas-liquid chromatography를 이용해서 분석했다. 또한 mouse에 화분립을 투여하였을 때 간장에서의 cholesterol치에 미치는 영향에 대해서도 조사하였다. 1. 화분립의 지질은 neutral lipid 84.10%, glycolipid 10.50%, phospholipid 5.40%를 함유했다. 2. 중성지방질의 조성은 triglyceride가 71.20%로 가장 많고, sterol ester, diglyceride, monoglyceride, free fatty acid, sterol의 순이다. 3. neutral lipid, glycolipid, phospholip의 주요지방산은 linoleic acid 28.48${\sim}$33.70%, palmitic acid 12.90${\sim}$47.50%, oleic acid 11.20${\sim}$12.20% 이다. 그러나 phospholipid는 다른 지질보다 palmitic acid를 더 많이 함유하고 있다. 4. 해바라기 화분립 투여 mouse의 체중은 대조군 보다 현저하게 증가했다. 5. 화분립을 투여한 mouse의 간장지질대사가 대조군보다 활발함을 알 수 있었다. 6. 화분립 투여 mouse의 간 cholesterol치가 초기에는 증가했으나, 말기에는 대조군과 비슷한 수준으로 감소했다.