Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 16 Issue 3
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- Pages.363-367
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- 1984
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- 0367-6293(pISSN)
Protoplast Formation and Regeneration of Streptococcus lactis
Streptococcus lactis의 Protoplast 생성 및 재생
- Cha, Sang-Hoon (Department of Food Engineering, Yonsei University) ;
- Shin, Won-Cheol (Department of Food Engineering, Yonsei University) ;
- Oh, Doo-Hwan (Department of Food Engineering, Yonsei University) ;
- Yu, Ju-Hyun (Department of Food Engineering, Yonsei University)
- Published : 1984.09.30
Abstract
Conditions for efficient formation and regeneration of protoplasts of Streptococcus lactis ATCC 11454 were investigated. Addition of 20mM DL-threonine into growth medium, growth phase and lysozyme concentration had significant effects on protoplast formation. Approximately, 20% regeneration efficiency was obtained by optimizing the medium composition and modifying the plating procedure.
Streptococcus lactis ATCC 11454 의 protoplast 생성 및 재생에 관하여 조사하였다. Protoplast의 생성은 용균효소로써 리소짐 단독처리 만으로 충분히 가능하였으며 균체의 배양은 20mM DL-트레오닌을 첨가한 배지를 사용하는 것이 좋았으며 생육시기와 리소짐 농도등이 중요한 영양인자였다. 배지조성의 최적화 및 protoplast를 배지에 접종하는 방법을 변형시킴으로써 약 20%정도의 재생효율을 얻을 수 있었다.
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