Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

A Simple and Modified Photometric Method for Measuring Lipase Activity

리파제 활성측정을 위한 간편한 비색정량법

  • Kim, Chul-Jin (Department Biological Science and Engineering, Korae Advanced Institute of Science and Technology) ;
  • Cheigh, Hong-Sik (Department of Food Science and Nutrition, Pusan National University) ;
  • Byun, Si-Myung (Department Biological Science and Engineering, Korae Advanced Institute of Science and Technology)
  • 김철진 (한국과학기술원 생물공학과) ;
  • 최홍식 (부산대학교 식품영양학과) ;
  • 변시명 (한국과학기술원 생물공학과)
  • Published : 1984.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A simple and modified method is developed to determine a lipase activity. A linear relationship exists between the color intensity and the free fatty acid liberated by enzyme action. The range of determination is from 0.05 to $1.5\;{\mu}moles$ of long chain fatty acid (oleic acid) and 0.2 to $2.0\;{\mu}moles$ of short chain fatty acid (caproic acid). The cumbersome procedure of the removal of the upper aqueous phase which was required in the previous copper soap extraction method was eliminated by the movement of solvent phase to upper phase in the respective biphasic system with a mixture solvent (chloroform: n-hexane:ethanol = 49:49:2) and copper reagent saturated with sodium chloride.

리파제 활성을 측정하는 간편한 방법을 개발하였다. 본 방법에 의하면 효소작용에 의해 생성된 유리지방산과 측정하는 색도와 서로 직선관계를 보였으며. 측정 범위는 long chain지방산인 경우 올레인산으로 환산하여 $0.05-1.5\;{\mu}moles$이었으며 short chain지방산인 경우 카프로인산으로 환산하여 $0.2-2.0\;{\mu}moles$의 범위였다. 본 방법의 장점은 종래의 "Copper Soap" 추출방법에서 상부 용매층 제거에 많은 시간을 소비하는 과정을 클로로포름-핵산-에타놀 혼합용매와 NaCl로 포화시킨 copper reagent를 사용하므로서 손쉽게 해결하였다.

Keywords