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Enzymatic Synthesis of Cephaloglycin

  • Doo-Hyun Nam (Department of chemistry, Korea Advanced Institute of Science and Technology) ;
  • Heon-Soo Sohn (Department of chemistry, Korea Advanced Institute of Science and Technology) ;
  • Dewey D. Y. Ryu (Department of chemistry, Korea Advanced Institute of Science and Technology)
  • 발행 : 1983.04.20

초록

Cephaloglycin was synthesized directly from D-${\alpha}$ -phenylglycine methyl ester and 7-aminocephalosporanic acid using whole cell enzyme of Xanthomonas citri (IFO 3835). Some optimal conditions for cephaloglycin synthesis were investigated, and yield improvements for its production by several methods were attempted. Using the whole cell enzyme system, the reaction kinetic model for cephaloglycin synthesis is proposed, and the kinetic constants for D-${\alpha}$ -phenylglycine methyl ester hydrolysis, cephaloglycin synthesis, and cephaloglycin hydrolysis were determined. The $K_m$ values of D-${\alpha}$-phenylglycine methyl ester, 7-aminocephalosporanic acid, and cephaloglycin were 11 mM, 24 mM, and 167 mM, and $K_i$ value of D-${\alpha}$-phenylglycine was 15 mM, respectively. The pattern of product inhibition was found to be competitive one.

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참고문헌

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피인용 문헌

  1. Reaction kinetics of cephalexin synthesizing enzyme from Xanthomonas citri vol.27, pp.7, 1983, https://doi.org/10.1002/bit.260270705