Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Purification and Characterization of Xylanase from Bacillus licheniformis,

Bacillus licheniformis Xylanase의 정제와 특성

  • Park, Yang-Do (Biotechnology Research Division, Korea Advanced Institute of Science and Technology) ;
  • Han, Moon-Hi (Biotechnology Research Division, Korea Advanced Institute of Science and Technology) ;
  • Kim, Jin-Mee (Biotechnology Research Division, Korea Advanced Institute of Science and Technology)
  • Published : 1983.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Three kinds of xylanases, X-C, X-I, and X-II, were separated from culture filtrate of an alkalophilic bacteria, Bocillus licheniformis OR-1. Their molecular weights were estimated to be 29, 000, 50, 000, and 34, 000, respectively. They were most active at pH 6.0-6.5, and at temperature of 5$0^{\circ}C$. Mercurc ion and p-chloromercurybenzoate inhibited the xylanase activity of X-C and X-II remarkably, whereas X-I was not affected. Xylanase X-I hydrolyzed barley straw xylan liberating xylose, xylobiose, and arabinose, while X-C and X-II produced only xylobiose and xylotriose.

Keywords