Journal of Environmental Health Sciences (한국환경보건학회지)
- Volume 9 Issue 1
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- Pages.89-93
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- 1983
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- 1738-4087(pISSN)
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- 2233-8616(eISSN)
Rapid Method for Seperation and Quantitation of p-Nitrophenol Derivative by HPLC
p-Nitrophenol 유도체의 HPLC에 의한 신속 분리 정량
Abstract
Various analytical methods for p-Nitrophenol derivatives have been reported as follows. 1) Thin-Layer Chromatography, 2) Gas Chromatography, 3) Cholinesterase Activity Determination, 4) Diazo Method, 5) Nitrophenol Method, 6) Indophenol Method. But these methods are mainly analyse total quantity of p-Nitrophenol and are not available for the seperation and pose some analytical problems associated with extensive clean up procedure. A rapid and simple method was developed for the seperation and quantitation for the p-Nitrophenol and it's derivatives by HPLC. Also an experiment was undertaken by the authors for the quantitation of the p-Nitrophenol in the blood of the intoxicated body. Levels of p-Nitrophenol ranging from approximately 0.10 to $1.69 \mu g/ml$ for Parathion and $3.44 \mu g/ml$ for EPN in each sample were measured with the average recovery of $95.5\pm0.52%$
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