Effect of Phospholipase D on the L-$\alpha$-Dimyristoyl-phosphatidyl Choline Liposome Containing Cholesterol, L-$\alpha$-Phosphatidylinositol and L-$\alpha$-Phosphatidylserine

Cholesterol, L-$\alpha$-Phosphatidylinositol, L-$\alpha$-Phosphatidylserine을 함유한 L-$\alpha$-Dimyristoyl-phosphatidyl Choline 리포솜에 대한 Phospholipase D의 작용에 관한 연구

When the reaction rate constant k of phospholipase D on liposome was measured by the ANS fluorometry, k of phospholipase D on DMPC liposome which was made of L-$\alpha$-PI, cholesterol and L-$\alpha$-PS decreased than that of phospholipase D on DMPC liposome with cholesterol or with PI and cholesterol. Optimal $Ca^{2+}$ concentration, the most important factor on effect of phospholipase D, also decreased to 1mM, as compared with 10mM and 60mM respectively when cholesterol and PI were added, and cholesterol only was added. The change of cholesterol Mol% had a great influence on k value of phospholipase D. But in case of addition of L-$\alpha$-PS to cholesterol, the influence was relatively diminished.