Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparative Studies on the Assay Methods of Stevia Sweeteners

스테비아 감미성분의 정량법에 관한비교

  • Kim, Nam-Soo (Food Research Institute, Agriculture and Fishery Development Corporation) ;
  • Oh, Sang-Lyong (Food Research Institute, Agriculture and Fishery Development Corporation) ;
  • Nam, Young-Jung (Food Research Institute, Agriculture and Fishery Development Corporation) ;
  • Min, Byong-Yong (Food Research Institute, Agriculture and Fishery Development Corporation) ;
  • Suh, Kee-Bong (Food Research Institute, Agriculture and Fishery Development Corporation)
  • 김남수 (농어촌개발공사 식품연구소) ;
  • 오상룡 (농어촌개발공사 식품연구소) ;
  • 남영중 (농어촌개발공사 식품연구소) ;
  • 민병용 (농어촌개발공사 식품연구소) ;
  • 서기봉 (농어촌개발공사 식품연구소)
  • Published : 1983.01.01
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Abstract

Analytical methods on Stevia sweeteners are compared for their reproducibilities and recoveries. It is possible to separate stevioside, rebaudioside A, rebaudioside C, and dulcoside A through HPLC analysis. Steviolbioside, in addition to above 4 Stevia sweeteners, is detected through TLC scanner and TLC-FID assays. C.V.s on stevioside and rebaudioside A in HPLC analysis are 1.39 and 4.89%, which shows outstanding reproducibilities of this method. The recoveries of stevioside in HPLC, TLC scanner, and TLC-FID analyses are 97.7 89.4, and 97.3%. The recoveries of rebaudioside A in HPLC, TLC scanner, and TLC-FID assays are 90.8, 90.1, and 75.8%. Total content of Stevia sweeteners in 8 strains tested, ranges from 5 to 17% as dry weight basis.

HPLC법으로는 stevioside, rebaudioside A, dulcoside A, rebaudioside C의 분리 정량이 가능하였고 TLC scanner법과 TLC-FID법상으로는 위의 4성분 외에 steviolbioside가 분리정량 되었다. HPLC 법에서 stevioside와 rebaudioside A에 대한 변이 계수는 1.39%와 4.89%로서 HPLC법의 높은 재현성을 보여주고 있다. HPLC, TLC scanner, TLC-FID법에서 stevioside의 회수율은 97.7, 89.4, 97.3%이고 rebaudioside A의 회수율은 90.8, 90.1, 75.8%였다. 분석시료 8종중 Stevia 감미성분의 전체 함량은 5%에서 17% 사이였다.

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