한국균학회지 (The Korean Journal of Mycology)
- 제11권2호
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- Pages.91-95
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- 1983
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
턱수염 버섯 배양(培養) 균사(菌絲)의 항종양(抗腫瘍) 성분(成分)
An Antitumor Constituent of the Cultured Mycelia of Hydnum repandum
- 정경수 (서울대학교 약학대학 미생물약품화학교실) ;
-
최응칠
(서울대학교 약학대학 미생물약품화학교실) ;
- 김병각 (서울대학교 약학대학 미생물약품화학교실) ;
-
김양섭
(농촌진흥청 농업기술연구소)
- Chung, Kyeong-Soo (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
-
Choi, Eung-Chil
(Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
- Kim, Byong-Kak (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
-
Kim, Yang-Sup
(Institute of Agricultural Sciences, O.R.D.)
- 발행 : 1983.07.30
초록
한국산 담자균류의 항종양성 성분을 탐색하기 위하여 턱수염버섯의 균사를 분리하여 인공배지에서 진탕배 양후, 그 배양균사체로부터 수용셩 단백성 다당류를 추출하였다. 이 성분이 ICR마우스에 이식한 육종 180에 대하여 54.3%의 종양억제율을 나타내었다. 이 성분은 다당류 42%와 단백질 28%로 구성되어 있었다. 그 중 다당류는 glucose (57.8%), mannose(19.3%), galactose(10.8%), xylose (6.8%), 및 fucose (5.7%)를 함유하고 있었다.
To investigate antitumor constituents of Hydnum repandum, which belongs to edible basidiomycetes, the mycelia separated from the carpophore were shake-cultured and subjected to hot water extraction. The extract was concentrated under vacuum and mixed with a three-fold volume of 95% ethanol to yield precipitates. The water soluble fraction of the precipitates was dialyzed and then lyophilized to yield a water soluble protein-polysaccharide fraction (= WPPF). This exerted antitumor activity against sarcoma 180 implanted in ICR mice. When administered i.p. at the dose level of 20mg/kg once daily for ten consecutive days, it showed an inhibition ratio of 54.3%. WPPF was found to be composed of a polysaccharide moiety (42% of WPPF) and a protein moiety (28% of WPPF) when determined by colorimetric method using anthrone reagent and Folin's phenol reagent. The polysaccharide moiety of WPPF was found to contain glucose (57.4%), mannose(19.3%), galactose (10.8%), xylose (6.8%), and fucose (5.7%), when the methanolysate of WPPF was analysed by GLC method.
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