Purification of Exo-dextranase from Aspergillus ustus

Aspergillus ustus가 생산하는 Exo-dextranase의 정제에 관한 연구

Aspergillus ustus was cultured in the salts media contained dextran (2%). Then the cultured liquid media were filtrated and concentrated up to 10 folds by evaporation, and then purified by means of acetone precipitation, of a repeated chromatography on the columns of DEAE-Ccellulose, Biogel P-150, and Sephadex G-200. Total proteins in the initial culture filtrate were 38,500mg, but the final amounts of proteins were 172mg. The specific activity of the protein in the culture filtrate was $1,340\;{\mu}moles$ products per minute per mg protein, but the final specific activity of the protein was $2,448\;{\mu}\;moles$ products per minute per mg protein. The final yields remained about 30% of the initial.

Aspergillus ustus의 dextranase을 정제하기 위하여 냉각 Aceton에 침전시키고, DEAE-cellulose, Bio-Gel P-150 및 Sephadex G-200을 사용하며 순수정제하였다. 처음 배양액 10l에 대한 총 효소량은 1,340kunits 단백질 양은 38,500mg였으며, 최종정제 단계인 Sephadex G-200에서는 총 효소 활성량은 421 kunits, 단백질량은 172mg이었으며 비활성은 약 70배로 증가했다.