Abstract
The wavelength of chlorophyll-b on the absorbance and fluorescence emission were shifted to the longer depending on the increasing of solvent polarities but fluorescence excitation spectra were not. The presence of chl-b oligomers and monomers were identified by the specra of fluorescence emission. Fluorescence excitation, absorbance and the measurement of its intensities vs. the concentration of n-prOH added to chl-b solution. The calibration curve of chl-b solution were not obeyed to Beer's law in the range of concentrated soln. because of the presence as the oligomers.
chl-b를 소중합체로서 용매에 녹였을 때 친핵성 n-prOH를 첨가함에 따라 흡광도와 fluorescence emission의 세기는 증가하였지만 단위체로 녹은 다음에 n-prOH의 농도증가에 따라 점차 감소하였다. chl-b는 소중합체의 생성때문에 Beer 법칙에 따르지 않았으며 용매의 극성변화에 따라 stockes shift를 나타냈다. 이러한 경향성은 chl-b의 정량에 고려하여야될 점이다.